Antisperm Antibody Test

Clinical definition of the Antisperm Antibody test
An antisperm antibody test is done to find antibodies against a man's sperm in blood, vaginal fluids, or semen. They can be produced either in a man's or woman's body.

Why is the Antisperm Antibody test done?
This test is done to find the reason of infertility when the couple is unable to produce children or to analyze the health of the reproductive organs. It is done as a blood test in females and as a sperm test in males. The antibodies can damage or kill sperm causing infertility. This is called immuno-logical infertility. Anti sperm antibodies can be made in the body of the man in the following cases:

  • When the testicles are injured
  • After surgeries (such as a biopsy or vasectomy)
  • To check the success of a vasectomy ( sterilization procedure for males)
  • After a prostate gland infection
  • Testicular cancer

A woman may have an allergic reaction to her partner’s semen, thus producing antibodies against it and causing infertility.

What are the common signs/symptoms when the Antisperm Antibody test is done?
Antisperm Antibody test is done when the couple might have a fertility problem and the woman is unable to conceive.

Who should do the Antisperm Antibody test (Target population)?

  • Couples suspected to have an infertility problem
  • As a follow up to a surgery such as a biopsy or a vasectomy

What should I do before the Antisperm Antibody test?

Specimen type

Specimen collection procedure

Preparatory instructions before the test *

For Women

Serum (Blood Sample)



No special preparations required.

For Men


Semen sample collection is through masturbation, directed into a clean cup.


Sperms must not be released or ejaculated for at least 2-5 days before the test.

If possible, do not avoid sexual activity for more than 1 to 2 weeks before the test, since a long period of sexual inactivity may result in less active sperm.

Avoid drinking alcohol for a few days before the test.

*Subjects suffering from any illness or on oral or injectable medications are advised to consult their physician prior to requesting any tests or procedures.

How do I interpret my Antisperm Antibody test results?

Reference Range*


00 to 60 U/ml **


Above 60 U/ml

Elevated values

** Units per ml
‘*A Reference range is a set of values which helps the healthcare professional to interpret a medical test. It may vary with age, gender, and other factors. Reference ranges may also vary between labs, in value & units depending on instruments used and method of establishment of reference ranges’

Diseases/conditions related to the Antisperm Antibody test

  • Twisting of the testicles
  • Injury to the testicles
  • Infection of the testis
  • Undescended testicles
  • Varicocele
  • Congenital Absence of the Vas Deferens (CAVD)
  • Testicular biopsy
  • Testicular cancer

Other tests related to the Antisperm Antibody test

  • FSH
  • LH
  • Testosterone
  • Free testosterone
  • Prolactin