Amylase (AMY)- serum

Clinical definition of the Serum Amylase test
The enzyme amylase plays a vital role in the digestion of carbohydrates. It is produced by the pancreas and the salivary glands. Disease, infection or inflammation of these glands causes the release of amylase into the blood or urine in increased levels.

Why is the Serum Amylase test done?
This test estimates the amount of the amylase enzyme in a patient's blood or urine sample. It is used to analyze the increasing or decreasing levels of amylase over time. It can also act as a biomarker to detect and monitor diseases of the pancreas such as Pancreatitis; formation of gallstones and some digestive tract problems.

What are the common signs/symptoms when the Serum Amylase test is done?

  • Severe abdominal or back pain
  • Fever
  • Anorexia
  • Nausea

Who should do the Serum Amylase test (Target population)?
People suffering from the above ailments or those who have had an injury of the pancreas.

What should I do before the Serum Amylase test?

Specimen type

Specimen collection procedure

Preparatory instructions during specimen collection*

Serum (Blood Sample)

Venipuncture- Collection of blood from a vein, usually from the arm.

The patient to be tested is strictly instructed not to drink any alcohol for about 24 hours before the test. If a blood amylase test is going to be done, the patient is advised not to eat or drink anything for at least two hours prior to the test.

*Subjects suffering from any illness or on oral or injectable medications are advised to consult their physician prior to requesting any tests or procedures.

How do I interpret my Serum Amylase test results?



20 to 330 units per liter (U/L)


Interpretive Data: Normally, only small amounts of amylase are found to be present in blood or urine. When the pancreas or salivary glands get damaged or their ducts get blocked, more amylase is released into the blood or urine.
‘*A Reference range is a set of values which helps the healthcare professional to interpret a medical test. It may vary with age, gender, and other factors. Reference ranges may also vary between labs, in value & units depending on instruments used and method of establishment of reference ranges’

Diseases/conditions related to the Serum Amylase test

  • Chronic pancreatitis
  • Diabetic ketoacidosis
  • Pancreatic pseudocyst
  • Cystic fibrosis
  • Acute pancreatitis
  • Cancer of the pancreas, ovaries, or lungs
  • Cholecystitis
  • Gallbladder attack resulting from disease
  • Infection of the salivary glands (such as mumps) or an obstruction
  • Intestinal obstruction
  • Macroamylasemia
  • Pancreatic or bile duct obstruction
  • Perforated ulcer
  • Tubal pregnancy (may be ruptured)
  • Viral gastroenteritis
  • Kidney disease
  • Toxemia of pregnancy

Other tests related to the Serum Amylase test

  • Lipase
  • Trypsin
  • Trypsinogen


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Amylase (Serum)